This DNA, known as the provirus, then integrates itself into the host cell's chromosomes. From Wordnik.com. [Aids The Next Ten Years] Reference
Since the general acceptance of the DNA provirus hypothesis in. From Wordnik.com. [Howard M. Temin - Autobiography] Reference
Sorbonne, with a thesis on «Lysogenic bacteria and the provirus concept». From Wordnik.com. [François Jacob - Biography] Reference
Often this results in permanent provirus status and incorporation to the genome. From Wordnik.com. [Of Prions and People - The Panda's Thumb] Reference
The provirus thus became a tool for studying regulation of DNA transcription in animal cells. From Wordnik.com. [Renato Dulbecco - Nobel Lecture] Reference
Here I performed the experiments that led in 1964 to my formulating the DNA provirus hypothesis. From Wordnik.com. [Howard M. Temin - Autobiography] Reference
SV40 (6), the viral DNA becomes a provirus, i.e. it establishes permanent, covalent bonds with the cellular DNA. From Wordnik.com. [Renato Dulbecco - Nobel Lecture] Reference
The trouble begins when the provirus starts directing enzymes in the host cell to produce new strands of viral RNA. From Wordnik.com. [Aids The Next Ten Years] Reference
The point that we are making is that we are in the beginning of an epidemic stemming from a provirus in our genomes. From Wordnik.com. [Invasion]
Opportunity No. 3 arises shortly after the provirus (the integrated viral DNA) starts running off RNA copies of itself. From Wordnik.com. [Aids The Next Ten Years] Reference
In that year, I performed the experiments that led to the formulation in the same year of the provirus hypothesis for Rous sarcoma virus. From Wordnik.com. [Howard M. Temin - Autobiography] Reference
It explained the persistence of the transformed state in the cell clone deriving from a transformed cell, since the provirus replicates with the cellular. From Wordnik.com. [Renato Dulbecco - Nobel Lecture] Reference
HIV-1 gene expression: lessons from provirus and non-integrated DNA. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
First, not all of the cells with a quiescent provirus respond to the treatment. From Wordnik.com. [Recently Uploaded Slideshows] Reference
The HIV-1Tg rat contains a gag-pol-deleted HIV-1 provirus regulated by a viral promoter. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
• Less than 1 cell per 1,000,000 resting CD4+ T cells from patients on HAART harbor latent HIV-1 provirus. From Wordnik.com. [Recently Uploaded Slideshows] Reference
HEK293T cells were co-transfected with 5 µg of pGCH provirus and 4 µg of either Tat-FLAG or Nullbasic plasmids. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
HEK293T cells were transfected with 5 µg of pGCH provirus and 4 µg or 8 µg of either Tat-FLAG or Nullbasic plasmids. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Figure 3C shows that provirus-expressed Gag levels were reduced in the presence of Nullbasic (lanes 4 and 5) compared to Tat-FLAG (lanes 2 and 3). From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
HERV are viruses that during replication the reverse transcriptase copies its retroviral RNA into the DNA of the host (it forms a haploid DNA provirus). From Wordnik.com. [ScienceBlogs Channel : Life Science] Reference
Nullbasic inhibits the RNA export function of provirus-expressed Rev. (A) Schematic diagram of the Rev-dependent CAT expression cassette in plasmid pDM128. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Virions were generated in HEK293T cells co-transfected with 1: 4 molar ratios of pGCH provirus to wild type Tat-FLAG, Nullbasic or empty vector ( "No Tat") plasmids. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
For the western blotting of cell lysates, HEK293T cells were transfected with either 5 µg of pGCH provirus, 1.5 µg of pGCH-Env-RTEm26CTE or 2 µg of pCMV5-Gag-FLAG. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Hypoacetylation of histone proteins by histone deacetylases (HDACs) is primarily involved in the maintenance of HIV-1 latency by repressing transcription from HIV-1 provirus. From Wordnik.com. [GEN News Highlights] Reference
Single LTRs frequently form by excision of internal proviral structure after homologous recombination between the two proviral LTRs, identical at the time of provirus integration. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
For the effect of Nullbasic on virion infectivity experiment, HEK293T cells were transfected with 5 µg of pGCH provirus and either 4 µg or 8 µg of Nullbasic or Tat-FLAG plasmids. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Host restriction factor APOBEC3G (A3G) blocks human immunodeficiency virus type 1 (HIV-1) replication by G-to-A hypermutation, and by inhibiting DNA synthesis and provirus formation. From Wordnik.com. [BioMed Central - Latest articles] Reference
To further examine the production of HIV-1 viral proteins, western blotting was performed on β-galactosidase-equalized lysates of cells co-expressing pGCH provirus and either Nullbasic or Tat-FLAG. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
HIV-1 virions from HEK293T cells co-transfected with 5 µg of pGCH provirus and 4 µg of either Tat-FLAG or Nullbasic plasmids were assayed for endogenous (intravirion) reverse transcription as previously described. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Odawara T, Oshima M, Doi K, Iwamoto A, Yoshikura H (1998) Threshold number of provirus copies required per cell for efficient virus production and interference in moloney murine leukemia virus-infected NIH 3T3 cells. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Vandergeeten C, Quivy V, Moutschen M, Van Lint C, Piette J, Legrand-Poels S: HIV-1 protease inhibitors do not interfere with provirus transcription and host cell apoptosis induced by combined treatment TNF-alpha + TSA. From Wordnik.com. [Recently Uploaded Slideshows] Reference
HIV-1 mRNAs isolated from HEK293T cells co-expressing pGCH provirus and either Tat-FLAG or Nullbasic were detected with a single probe capable of binding to the unspliced, singly-spliced and multiply-spliced classes of HIV-1 transcripts. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
(A) The infectivity of HIV-1 produced by HEK293T cells expressing pGCH provirus co-transfected with empty vector (pcDNA3. 1+) or co-expressing increasing amounts of Nullbasic or Tat-FLAG (at 1: 2 and 1: 4 molar ratios) were determined by the MAGI assay. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
"You're treating this virus like a provirus, which it is. From Wordnik.com. [Invasion]
B) HEK293T cells were co-transfected with pDM128 and pGCH provirus alone (column 2). From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
4) After a long, idle period, the provirus directs enzymes in the host cell to produce new strands of viral RNA. From Wordnik.com. [Aids The Next Ten Years] Reference
When the pGCH provirus was used to provide Rev in the reporter assay, co-expression of Nullbasic resulted in a statistically-significant decrease (. From Wordnik.com. [PLoS ONE Alerts: New Articles] Reference
Integration: The provirus. From Wordnik.com. [Renato Dulbecco - Nobel Lecture] Reference
This integrated DNA is called provirus. (. From Wordnik.com. [Aids The Next Ten Years] Reference
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