Creation of pig-sheep xenogeneic chimerism by the in-utero transplantation of hematopoietic stem cells.    From Wordnik.com.  [Fetal Stem Cell Transplantation, In Utero Stemm Cell Publications] Reference

Rice HE, Hendrick MH, Flake AW: In-utero transplantation of rat hematopoietic stem cells induces xenogeneic chimerism in mice.    From Wordnik.com.  [Fetal Stem Cell Transplantation, In Utero Stemm Cell Publications] Reference

Rice HE, Flake AW, Hedrick MH, Zanjani ED, Harrison MR: Effect of xenogeneic chimerism in a human/sheep model on natural antibody.    From Wordnik.com.  [Fetal Stem Cell Transplantation, In Utero Stemm Cell Publications] Reference

In a model of xenogeneic graft-versus-host disease.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

A xenogeneic transplantation model utilized Rag2 −/− γc.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Leukemia xenogeneic 2: Donor - human ALL / AML BM; Recipient.    From Wordnik.com.  [Recently Uploaded Slideshows] Reference

Tregs or Treg-conditioned DC protect against lethal xenogeneic GVHD.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Leukemia xenogeneic 1: human CD34+ transplant (before or after) in leukemic Nalm-6-GFP ALL 7.    From Wordnik.com.  [Recently Uploaded Slideshows] Reference

Our initial xenogeneic GVHD experiments utilized PBMC or purified lymphocytes as the human T cell inocula.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Next, we evaluated whether human Treg and Treg-conditioned DC might modulate xenogeneic GVHD in a PD-L1-dependent manner.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Previous xenogeneic GVHD models have utilized human peripheral blood mononuclear cells (PBMCs) that contain unmanipulated human T cells.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Zanjani ED, Pallavacini MG, Ascensao JL, Flake AW, Harrison MR, Tavassoli M.: Human-ovine xenogeneic transplantation of stem cells in-utero.    From Wordnik.com.  [Fetal Stem Cell Transplantation, In Utero Stemm Cell Publications] Reference

Next, we utilized an in vivo xenogeneic transplantation model to further characterize the ability of Tregs or Treg-conditioned DC to modulate the PD1 pathway.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Subsequent experiments were designed to identify human inocula that yielded enhanced human T cell engraftment and a resultant increase in lethal xenogeneic GVHD incidence.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Critically, NOD-scid mice enable xenogeneic transplantation without rejection/immunosuppressant drug administration, and allow achievement of 100% engraftment not seen in other models.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Pallavicini MG, Flake AW, Bethel C, Langlois R, Madden D, Duncan BW, Haendel S, Montoya T: Creation of human-mouse xenogeneic chimeras by the in-utero transplantation of hemopoietic cells.    From Wordnik.com.  [Fetal Stem Cell Transplantation, In Utero Stemm Cell Publications] Reference

Further in vivo experiments were performed to evaluate the effect of Tregs and Treg-conditioned DC on human T cell engraftment, cytokine activation, and induction of lethal xenogeneic GVHD.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

It should be stated that xenogeneic models of GVHD likely do not fully reflect the biology of clinical GVHD, and as such, the potential clinical implications of the findings in our model must be interpreted with caution.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Most importantly, we have significantly extended this prior work through our discovery that myeloid DC conditioned by Tregs were effective in vivo for the complete elimination of posttransplant lethal xenogeneic GVHD induced by effector T cells.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

These models are limited by their xenogeneic nature, whereas the DR4 mice described here provide cases of spontaneous disease with morphological and phenotypic similarities to human T cell/histiocyte rich DLBCL and HL with characteristic H/RS-like cells.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

It is interesting to note that the Treg-conditioned DC did not express significant PD-1; it is thus possible that the capacity of this cell population to effectively prevent xenogeneic GVHD may reside in part on a limited susceptibility to PD-1-mediated suppression.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

It is perhaps important to emphasize that the regulatory T cell or Treg-conditioned DC modulation of xenogeneic GVHD was robust because it occurred at the relatively low regulatory cell to effector cell ratio of 1: 20, which is considered to represent a physiologic ratio.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Specifically, the xenogeneic transplantation model that we utilized did not incorporate a human hematopoietic stem cell component, and as such, the potential effect of the regulatory T cells or Treg-conditioned DC that we evaluated on stem cell engraftment was not assessed.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Recipients of Tregs or Treg-conditioned DC were uniformly protected against lethal xenogeneic GVHD (.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Figure 5A (i)); importantly, recipients of control CD4-conditioned DC uniformly died of xenogeneic GVHD.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Posttransplant weight loss, which is a more sensitive clinical parameter of xenogeneic GVHD, was moderated by Treg-conditioned DC therapy and virtually eliminated by Treg therapy (.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

In addition to this apparent DC-mediated mechanism of GVHD protection, other non-APC mechanisms are likely operative for the Tregs that we studied, because transplant cohorts that received Tregs had more robust protection against xenogeneic GVHD than recipients of Treg-conditioned DC (lowest CD4.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

In a second experiment, we confirmed the ability of Treg-conditioned DC to completely abrogate the generation of lethal xenogeneic GVHD; importantly, protection against lethal xenogeneic GVHD conferred by the Treg-conditioned DC was completely abrogated by anti-PD-L1, but not by isotype control antibody (.    From Wordnik.com.  [PLoS Biology: New Articles] Reference