Sigma. taking a chance that the cDNA sequence had derived from a single exon.    From Wordnik.com.  [Kary B. Mullis - Nobel Lecture] Reference

Checked on the PCR primers, decided it is probably the cDNA prep that is bad.    From Wordnik.com.  [See: Fingernails, holding on by] Reference

Image used with permission from Clontech. pDsRed: This is a cDNA copy of the RFP gene (DsRed).    From Wordnik.com.  [Archive 2005-10-01] Reference

And what came up was Douglas Prasher's paper about the cloning of the cDNA for green fluorescent protein.    From Wordnik.com.  [Martin Chalfie - Interview] Reference

In 1992, the cDNA of GFP was cloned by Dr. Douglas Prasher, who was then at Woods Hole Oceanographic Institution.    From Wordnik.com.  [Osamu Shimomura - Autobiography] Reference

Greg Preston, a Postdoctoral Fellow with strong molecular biological experience, cloned the cDNA for the 28 kDa protein.    From Wordnik.com.  [Peter Agre - Autobiography] Reference

Nor are they specific utilities, because they could be asserted for any partial cDNA transcribed from any gene in the human genome.    From Wordnik.com.  [Archive 2008-02-01] Reference

I was building a cDNA library and would occasionally have to show up in the middle of the night to check my colonies or adjust the broth.    From Wordnik.com.  [Interference pattern] Reference

Human BIN1 (Isotype 8) cDNA was obtained from Origene.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

Each set of primers bridged exons to ensure amplification of cDNA only.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Full-length cDNA corresponding to BiP3 was amplified by PCR with primers.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Lipofectamine (Invitrogen) was used for cDNA transfections in HL-1 cells.    From Wordnik.com.  [PLoS Biology: New Articles] Reference

The zebrafish Ugt cDNA clones and their GenBank accession numbers are shown.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

However, in the presence of ONNV (+) strand cDNA, the slope was − 1.4, the.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

FOXL2 cDNA was cloned into pGBKT7 vector, and transformed into AH109 yeast cells.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

SUMO-1 cDNA was derived from a pGEX2T.SUMO-1 vector kindly provided by Ronald T. Hay.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Multiple attempts to clone the cDNA for allergen Asp f16 were made, without success.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Full length MITF-M and its splice variant cDNA were cloned from human melanoma cell line.    From Wordnik.com.  [BioMed Central - Latest articles] Reference

Each small RNA cDNA library was denatured with 2 N NaOH to a final concentration of 0.5 nM.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Firstly 10 ng of total RNA were reverse transcribed into cDNA using the miRNA-specific primer.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Human full length PIAS1 and UBC9 cDNA obtained from pACT2 vectors isolated by two hybrid screen, and.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Pairs of differentially labeled cDNA samples from each time point were hybridized to a custom Agilent.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Only at dilutions in which ONNV (−) strand cDNA was present in excess of ONNV (+) strand cDNA were C.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

These assays incorporate a unique tag sequence into cDNA synthesized from a specific strand of viral RNA.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Chen TB, Lu HH, Lee YS, Lan HJ (2008) Segmentation of cDNA microarray images by kernel density estimation.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Only heteroduplexes that disanneal at low stringency are used for constructing the resulting cDNA library.    From Wordnik.com.  [BioMed Central - Latest articles] Reference

RT-PCR was performed for both THBS2 (liver) and SMOC2 (liver) in order to define the cDNA structure of these genes.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

An equal amount of RNA was reverse transcribed to generate the cDNA template to be used in qRT-PCR reactions using oligodT.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

(Invitrogen). cDNA was synthesized from these samples using the SuperScript III First-Strand Synthesis System (Invitrogen).    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Total RNA was extracted and used as template for cDNA synthesis using a SuperScript III Reverse Transcription Kit (Invitrogen).    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Oligonucleotides designed with Asp f9 sequence information from TIGR only yielded Asp f9 cDNA PCR amplicons from ATCC 42202 cDNA.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

To look for proteins interacting with FOXL2, we initially performed a yeast two hybrid assay to screen a human ovary cDNA library.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

However, the amount of cDNA quantified during qPCR must accurately reflect amounts of a specific viral RNA strand in the RT reaction.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Primer was added to the reverse transcription reaction at a final concentration of 500 nM. cDNA was synthesized with Superscript II®.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Serial dilutions of cDNA generated from in vitro transcribed CHIKV RNAs were used to confirm strand-specificity and determine sensitivity.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference