Ulmer settled on a method of sequencing single molecules of DNA, using an enzyme called exonuclease to nibble off bases from an unfurled DNA strand like a PacMan video game.    From Wordnik.com.  [The $1,000 Genome] Reference

"Excision" in this case refers to excision by endonucleases or glycosylases, not nicking + exonuclease as in mismatch repair.    From Wordnik.com.  [Another Protozoan and Front-Loading] Reference

DNA polymerase, Pol I, extends the DNA from the free 3'-OH using its exonuclease activity to replace the nucleotide of the damaged base, as well as a few downstream, followed by sealing of the new DNA strand by DNA ligase.    From Wordnik.com.  [Another Protozoan and Front-Loading] Reference

The idea was to attach an exonuclease to the lip of the nanopore: the enzyme would snip the end of an incoming strand of DNA like a PacMan, one base at a time.31 Each newly freed base then tumbles into the nanopore for its identity to be revealed by the transient blockage in current.    From Wordnik.com.  [The $1,000 Genome] Reference

It contains a C-terminal nuclease domain with homology to the endonuclease-exonuclease-phosphatase (EEP) family of enzymes.    From Wordnik.com.  [Naturejobs - All Jobs] Reference

•We also demonstrate a second method for in vitro mutagenesis following treatment of PCR fragments with lambda exonuclease.    From Wordnik.com.  [Recently Uploaded Slideshows] Reference

The stimulation of Fen1 by Mph1 was limited to flap-structured substrates; Mph1 hardly stimulated the 5 'to 3' exonuclease activity of Fen1.    From Wordnik.com.  [Journal of Biological Chemistry current issue] Reference

They include genes coding for proteins involved in DNA repair and modification as RC0550 (RecB exonuclease), RC1204 (tmRNA-binding protein), RC1050.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

The gene responsible for WS encodes a DNA helicase/exonuclease protein believed to affect different aspects of transcription, replication, and/or DNA repair.    From Wordnik.com.  [BioMed Central - Latest articles] Reference

The fluorescent reporter dye is released from the 5′ end of the probe by the exonuclease activity of Taq polymerase, reducing proximity to a quencher dye at the 3′ end of the probe.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Nature Nanotechnology paper by showing that the nanopore does indeed work in salt conditions favoring exonuclease activity and can detect bases cleaved off DNA strands in solution by the enzyme.    From Wordnik.com. Reference

Holland PM, Abramson RD, Watson R, Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′ ---- 3′ exonuclease activity of Thermus aquaticus DNA polymerase.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

They go on to state: "These advances represent the realization of a complete nanopore base detector, which, when combined with a compatible exonuclease DNA processing system, will provide the basis of a complete nanopore sequencer.".    From Wordnik.com. Reference

That indeed is the next crucial step for Oxford Nanopore - to couple an exonuclease enzyme to the nanopore so that single bases are cleaved off a template DNA strand and their identity read off instantaneously as they funnel through the nanopore.    From Wordnik.com. Reference

Following amplification, samples were digested for 30 minutes at 37°C with 10 U lambda exonuclease (NE Biolabs, Ipswich, MA.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

Unincorporated primers present in heat inactivated reverse transcription reactions were digested with exonuclease I (New England Biolabs). cDNAs used in the generation of standard curves were serially diluted (~10.    From Wordnik.com.  [PLoS ONE Alerts: New Articles] Reference

exonuclease enzyme, 81, 176, 207, 227, 244.    From Wordnik.com.  [The $1,000 Genome] Reference