That in the same system one can with RIA measure both an enzyme and its proenzyme and other inactive forms has both advantages and disadvantages, depending on the problem under investigation. From Wordnik.com. [Nobel Lecture Radioimmunoassay: A Probe For Fine Structure Of Biologic Systems] Reference
TPP1 is synthesized as an inactive proenzyme (pro-TPP1) that is proteolytically processed into the active enzyme after exposure to low pH in vitro or targeting to the lysosome in vivo. From Wordnik.com. [Journal of Biological Chemistry current issue] Reference
The proenzyme structure reveals suboptimal catalytic triad geometry with its propiece linker partially blocking the substrate-binding site, which together serve to prevent premature activation of the protease. From Wordnik.com. [Journal of Biological Chemistry current issue] Reference
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